RecA binding to bulge- and mismatch-containing DNAs. Certain single base mismatches provide strong signals for RecA binding equal to multiple base bulges
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چکیده
منابع مشابه
recA Protein Binding to the Heteroduplex Product
Following DNA strand exchange, recA protein remains associated with the heteroduplex DNA product of the reaction. This association exists without significant changes (as measured by nuclease protection, topological state of the heteroduplex DNA, and rates of ATP hydrolysis) for at least 30 min after strand exchange is complete. The heteroduplex DNA is unwound as a result of this association to ...
متن کاملGeneral mechanism for RecA protein binding to duplex DNA.
RecA protein binding to duplex DNA occurs by a multi-step process. The tau analysis, originally developed to examine the binding of RNA polymerase to promoter DNA, is adapted here to study two kinetically distinguishable reaction segments of RecA-double stranded (ds) DNA complex formation in greater detail. One, which is probably a rapid preequilibrium in which RecA protein binds weakly to nati...
متن کاملSingle base bulges in small RNA hairpins enhance ethidium binding and promote an allosteric transition.
A set of four RNA hairpin helices has been prepared by in vitro transcription with T7 RNA polymerase. The hairpins all contain the same nine base pair helix, but with an extra A, C, or U residue forming a bulge at one position; the fourth hairpin is a perfect helix with no bulge. The helix with a bulged A duplicates six base pairs of a helix in the 16S rRNA known to have an unusually high affin...
متن کامل1H NMR determination of base-pair lifetimes in oligonucleotides containing single base mismatches.
Proton nuclear magnetic resonance (NMR) spectroscopy is employed to characterize the kinetics of base-pair opening in a series of 9mer duplexes containing different single base mismatches. The imino protons from the different mismatched, as well as fully matched, duplexes are assigned from the imino-imino region in the WATERGATE NOESY spectra. The exchange kinetics of the imino protons are meas...
متن کاملPurine Binding to Dinucleotides: Evidence for Base
* This investigation was supported in part by U.S. Public Health Service research grant GM 12555, USPHS training grant TG 722, and National Science Foundation grant NSF GP 700. 1 Vinograd, J., J. Lebowitz, R. Radloff, R. Watson, and P. Laipis, these PROCEEDINGS, 53, 1104 (1965). 2 Crawford, L. V., and P. H. Black, Virology, 24, 388 (1964). 3 Jansz, H. S., and P. H. Pouwels, Biochem. Biophys. Re...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)85371-1